Description
The Eagle Biosciences Mouse Interleukin 1 Beta (IL-1β) ELISA Assay Kit employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for Interleukin 1 Beta (IL-1β) has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Interleukin 1 Beta (IL-1β) present is bound by the immobilized antibody. Following incubation unbound samples are removed during a wash step, and then a detection antibody specific for Interleukin 1 Beta (IL-1β) is added to the wells and binds to the combination of capture antibody-IL-1β in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps a substrate is added. A colored product is formed in proportion to the amount of Interleukin 1 Beta (IL-1β) present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven Interleukin 1 Beta (IL-1β) standard dilutions and Interleukin 1 Beta (IL-1β) sample concentration determined